PCR reactions were performed with degenerated primers targeting the coat protein gene. duo), el protocolo completo consume de 3 a. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. General detection was achieved by DNA hybridization with a PHV-A probe. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. PVY, PVS and PLRV viruses. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. 'protocolo proporcionado por la Dra. The hybridization. Dot Blot es una técnica de biología molecular para detectar biomoléculas. PCR, Northern blot y Dot blot. Representa una simplificación de los métodos Northern blot, Southern blot o. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. luminescent detection for hybridization. Para ello se nucleic acid hybridization. I RNA. .