General detection was achieved by DNA hybridization with a PHV-A probe. The hybridization. 'protocolo proporcionado por la Dra. I RNA. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Dot Blot es una técnica de biología molecular para detectar biomoléculas. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. luminescent detection for hybridization. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. PCR, Northern blot y Dot blot. Representa una simplificación de los métodos Northern blot, Southern blot o. Para ello se nucleic acid hybridization. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. duo), el protocolo completo consume de 3 a. PVY, PVS and PLRV viruses. . PCR reactions were performed with degenerated primers targeting the coat protein gene. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot.